Product name: Light Stabilizer 783
Information: COA, MSDS, TDS
Two-component synergistic effect: HALS944 + HALS622 are mixed together
Shelf life: 24 months
Sulforhodamine B CAS 3520-42-1
Sulforhodamine B CAS 3520-42-1
Sulforhodamine B, with CAS number 3520-42-1, is highly soluble in water (solubility at 20°C is 95.3g/L), and can also dissolve in methanol and ethanol. The aqueous solution appears blue-red and has a bright yellow fluorescence; it turns orange-yellow in concentrated sulfuric acid and turns red after dilution. It contains two sulfonic acid groups, has strong hydrophilicity, is a non-permeable fluorescent tracer dye for cell membranes, and can quantitatively combine with basic amino acids of proteins under weak acid conditions.
Appearance: Deep red to purplish red crystalline powder
Spectral parameters: Maximum absorption λmax = 554 nm
Emission λem = 584 nm; molar extinction coefficient ≥ 8500
Specification
| Items | Specification |
| Appearance | Deep red to purplish red crystalline powder |
| Density | 1.472 [at 20℃] |
| Solubility in methanol | 1 mg/mL |
| Color index | 45100 |
Application
1. Biology: SRB cytotoxicity/cell proliferation assay, fluorescence tracing, tissue staining, laser dyes;
2. Industrial dyes: Wool, silk, nylon acid dyeing, leather, paper coloring, fluorescent coloring.
✨ Product Features
1. Excellent water solubility: The bisulfonic acid groups provide strong hydrophilicity, resulting in stable aqueous solutions, simple preparation, and no need for solubilizers.
2. Strong fluorescence signal, high molar extinction coefficient, high detection sensitivity, and good signal-to-noise ratio.
3. Quantitative control of protein binding: Binding to proteins under acidic conditions, and elution under alkaline conditions; stable signal, long-term preservation after fixation staining, unlike MTT which is susceptible to metabolic interference.
4. Superior light stability compared to common rhodamines, slow fluorescence decay under long-term illumination, suitable for long-term imaging and laser dye applications.
5. Membrane impermeability: Cannot penetrate the living cell membrane, suitable for extracellular tracing, vascular/liquid tracing.
6. Bright coloring with fluorescence, good uniformity of staining, can obtain bright fluorescent red for wool/textile dyeing.
7. Wide pH tolerance range, excellent stability in neutral and weakly acidic environments in aqueous solutions.
Packaging and Storage
25kg cardboard drums, or 25kg composite kraft bags.
Short-term storage in warehouse: ≤25℃, protected from light, dry and ventilated, away from direct sunlight.
Long-term high-purity research grade: Recommended refrigeration at 2~8℃; for ultra-long-term storage, it is recommended to store at -20℃ in a sealed manner, with better fluorescence stability.
Warehouse stacking: Place on raised pallets, avoid direct contact with the ground to prevent moisture; strictly prohibit mixing with strong oxidants, strong acids and strong bases.
FAQ
Q: Why must protect from light?
A: SRB is light sensitive. Continuous light exposure will reduce fluorescence intensity and fade color, leading to inaccurate test results for biological assays.
Q: Is the powder hygroscopic?
A: Slightly hygroscopic. Seal tightly to prevent moisture. Mild caking does not affect performance after dissolution.
Q: Packaging options?
A: Industrial grade: 25kg fiber drum with inner light-proof PE bag. Pallet shipment available.
Q: MOQ & sample service?
A: Small testing samples available.
Q: What documents can you provide?
A: COA, MSDS, TDS, spectrum data.