Unlock the amazing biocatalyst in industry-Laccase

Laccase CAS 80498-15-3 is a copper-containing polyphenol oxidase belonging to the cupric blue oxidase family, also known as Novozyme 809. It is widely distributed in the biological world, from plants and fungi to insects and even prokaryotes. In 1883, Japanese scholar Yoshida first discovered Laccase CAS 80498-15-3 from sumac liquid, this enzyme is found in sumac resin, hence the name. Later, people found Laccase from a large number of fungi, basidiomycetes in the white rot is the main source of Laccase CAS 80498-15-3. Lassase exists in the form of a monomeric glycoprotein, and its catalytic center contains clusters of 4 copper atoms, which play a key role in the catalytic process. Laccase CAS 80498-15-3 catalyzed reaction is the use of molecular oxygen, the substrate for single electron oxidation, generate the corresponding active free radicals, the whole process, 1 molecule of oxygen is reduced to 2 molecules of water, and the only by-product is only water, this feature makes it in the field of environmental protection has a unique advantage.

The substrate range of Laccase CAS 80498-15-3 is very broad, covering many substances such as phenols, aromatic and fatty amines. The structure and catalytic properties of Laccase CAS 80498-15-3 from different sources may vary, even if the same source, like a white rot strain, may secrete different properties of Laccase components, including oxidation capacity, optimal pH, substrate specificity, etc. It is these differences that make Laccase CAS 80498-15-3 show diverse application potential in different fields.

△ Application of Laccase CAS 80498-15-3 in paper industry

1.Decompose lignin to improve paper texture

Lignin is an important component of plant cell wall and widely exists in papermaking raw materials. However, in the papermaking process, too much lignin will affect the quality of the paper. The traditional method of lignin removal is mainly chemical method, and the commonly used chemical agents such as chlorine gas and sodium hydroxide can effectively remove lignin, but it also brings a series of serious problems. These chemicals will produce a lot of waste water containing harmful substances in the process of use, which is not only difficult to degrade, but also cause long-term pollution to soil and water sources, endangering the ecological environment and human health.

Laccase CAS 80498-15-3 provides a new way to solve this problem. Laccase CAS 80498-15-3 can specifically catalyze the degradation of lignin. Its principle of action is based on its unique structure and catalytic properties. The Laccase CAS 80498-15-3 molecule contains multiple copper ions, which form specific active centers. When Laccase CAS 80498-15-3 is in contact with lignin, copper ions seize electrons from lignin molecules through REDOX reaction, so that lignin molecules are oxidized to form free radical intermediates. These free radical intermediates further undergo a series of chemical reactions, such as oxidation, cracking, etc., and finally decompose the lignin into small molecules, so as to achieve the removal of lignin.

Compared with traditional chemical methods, Laccase decomposition of lignin has obvious advantages. First of all, Laccase reaction conditions are mild, generally at room temperature, atmospheric pressure and near neutral pH conditions can be carried out, which greatly reduces the requirements for equipment, reducing energy consumption. Second, Laccase CAS 80498-15-3 has a high degree of selectivity, it only acts on lignin, and does not cause damage to other components such as cellulose, which effectively retains the integrity and strength of cellulose, and improves the physical properties of paper. The strength and durability of the paper treated with Laccase CAS 80498-15-3 are significantly improved, and the paper is more durable and durable, which can meet the needs of higher quality printing and writing.

2.Help pulp bleaching, open a new chapter of environmental protection

Pulp bleaching is a key step in the papermaking process, its purpose is to remove the pigment and lignin in the pulp and improve the whiteness and brightness of the paper. The traditional bleaching methods of pulp mainly include chlorine bleaching, hypochlorite bleaching, etc. Chlorine bleaching Although the bleaching effect is significant, the cost is relatively low, but chlorine is a toxic gas, there is a risk of leakage during the production process, posing a threat to the health of the operator. Moreover, chlorine bleaching will produce a large number of chlorine-containing organic compounds, which have strong toxicity and carcinogenicity, and will cause serious pollution to the environment. Hypochlorite bleaching also presents a similar problem, producing organochlorides, which are not friendly to the environment, but also tend to damage the fiber, affecting the strength and durability of the paper.

Laccase bleaching is a more environmentally friendly and gentle bleaching method. The principle of Laccase bleaching is to use the oxidation of Laccase to decompose the pigment and lignin in the pulp, so as to achieve the purpose of bleaching. In the Laccase CAS 80498-15-3 bleaching process, Laccase CAS 80498-15-3 first reduces oxygen to water while oxidizing substrates (pigments and lignin) to free radical intermediates. These free radical intermediates further react to form a colorless product, thus achieving bleaching of the pulp. Compared with traditional bleaching methods, Laccase bleaching has many advantages. It does not require the use of a large number of chemical agents, greatly reducing the pollution of chemical substances to the environment. The damage of Laccase CAS 80498-15-3 to the fiber is small, which can better retain the original properties of the paper, so that the whiteness of the paper is more stable and the strength is higher.

△Application of Laccase CAS 80498-15-3 in textile printing and dyeing

1.Fiber modification, giving the fabric new properties

In the textile field, natural fibers such as wool, cotton, ramie, etc., have always been the preferred materials for making high-quality fabrics. However, there are some limitations in the performance of these natural fibers, such as wool is easy to felting and uneven dyeing, cotton’s hygroscopic and dyeing properties need to be improved, ramie fiber is more rough and poor feel. Traditional fiber modification methods mainly rely on physical or chemical means, such as the use of chemical agents for wool surface treatment, although can improve fiber performance to a certain extent, but these chemical agents are often unfriendly to the environment, easy to cause pollution. Moreover, when using protease and other biological enzymes for modification, it is easy to cause excessive damage to the fiber, affecting the strength and durability of the fiber.

Laccase CAS 80498-15-3 provides a gentler, more environmentally friendly and more efficient solution for fiber modification. The modification effect of Laccase CAS 80498-15-3 on natural protein fibers, such as wool, is particularly significant. Laccase CAS 80498-15-3 can catalyze some chemical reactions on the surface of wool, making the surface of wool fiber more smooth, thereby reducing friction between fibers and reducing the occurrence of felting phenomenon. Laccase CAS 80498-15-3 can also improve the dyeing properties of wool, so that the dye is easier to dye, dyeing is more uniform, and the color depth is increased. The researchers used Laccase CAS 80498-15-3 to modify the wool fiber, and found that the scale structure of the surface of the modified wool fiber became more flat, and the wetting time was greatly shortened, which meant that the moisture absorption of the wool was improved, and it would be more comfortable to wear. Moreover, in the dyeing process, the utilization rate of dyes is significantly improved, reducing the waste of dyes, and also reducing the difficulty of dyeing wastewater treatment.

2.Dyeing and decolorization, environmentally friendly and efficient

In the dyeing process, Laccase CAS 80498-15-3 triggers phenolic polymerization dyeing, which brings a new idea for textile printing and dyeing. Traditional dyeing methods, mostly use synthetic dyes, these dyes in the production and use process, often consume a lot of water resources and chemical additives, and some synthetic dyes are difficult to degrade, will cause pollution to the environment. Laccase CAS 80498-15-3-catalyzed staining has unique advantages. Laccase CAS 80498-15-3 can trigger the polymerization of phenolic substances to produce colored polymers, which can be used to dye fabrics. Common phenolic substances, such as gallic acid, tea polyphenol, catechol, hydroquinone, etc. are polymerized under the action of Laccase CAS 80498-15-3 to form colored polymers with good dyeing properties. They can not only give the fabric good dyeing depth and dyeing fastness, but also make the fabric have special properties such as antibacterial. Moreover, this dyeing method is environmentally friendly, does not require the use of a large number of chemical additives, reduces the discharge of wastewater, and is in line with the concept of sustainable development.

△Application of Laccase CAS 80498-15-3 in food industry

1.Used as food additive to enhance food value

In the field of food additive production, Laccase CAS 80498-15-3 can transform ordinary substances into food additives with high added value. Tea polyphenols and anthocyanins, which have antioxidant and anti-inflammatory effects, can be efficiently synthesized under the catalysis of Laccase CAS 80498-15-3. Laccase CAS 80498-15-3 can also be used to produce vanillin, a compound with a strong vanilla aroma that adds a unique flavor to foods and enhances their taste and appeal. In baked goods, the addition of vanillin can make bread, cake and other attractive aroma, so that people’s appetite increase. The application of Laccase CAS 80498-15-3 in fatty foods shows its powerful antioxidant effect. Fat is prone to oxidative rancidity during storage, resulting in unpleasant odor and harmful substances, which affect the quality and safety of food. Add an appropriate amount of Laccase CAS 80498-15-3 to the oil food, Laccase CAS 80498-15-3 can oxidize some ingredients in the food, produce antioxidant effect, so as to effectively extend the shelf life of the oil. The experimental data showed that the oxidation induction period of Laccase CAS 80498-15-3 was significantly prolonged and the antioxidant properties were significantly improved, which provided a strong guarantee for the long-term storage and sale of fatty food.

2.Optimize the processing process to improve food quality

In the process of brewing, it plays a key role in improving the quality of beer. Laccase CAS 80498-15-3 can catalyze the oxidative polymerization of phenolic substances in wort to form larger molecular particles, which are easier to be removed by filtration and improve the clarification of wort.

In the process of tea making, the color and taste of tea are important indicators to measure the quality of tea. Laccase CAS 80498-15-3 can change the structure of pigment components and polyphenols in tea through oxidation, so as to improve the color and taste of tea. For green tea, Laccase CAS 80498-15-3 can promote the oxidation of tea polyphenols, make the color of tea more green, while reducing bitter taste, increase fresh feeling. For black tea, Laccase CAS 80498-15-3 contributes to the deep oxidation of tea polyphenols, forming more theaflavins and thearubin, making tea more rosy in color and more mellow in taste. Laccase CAS 80498-15-3 also plays an important role in the production of beverages such as fruit and vegetable juices. It can decompose pectin and other macromolecular substances in the beverage, reduce the viscosity of the beverage, improve the clarification, make the beverage more clear and transparent, and taste more delicate.

△Laccase CAS 80498-15-3 plays an important role in the field of environmental protection

Laccase CAS 80498-15-3 has shown remarkable ability in wastewater treatment. It can decompose benzene, phenols, dyes and other organic matter in wastewater into small molecular substances through its unique catalytic effect, so that its toxicity is reduced, and even converted into harmless substances. The catalytic principle of Laccase CAS 80498-15-3 is based on the copper ions in its molecular structure. In the catalytic process, copper ions seize electrons from organic molecules through REDOX reaction, so that organic molecules are oxidized to form free radical intermediates. These free radical intermediates further react, such as oxidation, cracking, etc., and finally decompose organic matter into small molecules of carbon dioxide, water and other substances.

Laccase CAS 80498-15-3 produced by unilong was mainly fermented by microorganisms. In the fermentation process, strains, medium components and fermentation conditions were optimized to improve the yield and activity of Laccase CAS 80498-15-3. You need to click to get a good price.