MES Monohydrate with CAS 145224-94-8 - Image 2

MES Monohydrate with CAS 145224-94-8

CAS:145224-94-8
Molecular Formula:C6H15NO5S
Molecular Weight:213.25
Appearance: White crystalline powder
EINECS:224-632-3
Synonyms:2-(4-morpholinyl) ethanesulfonic acid hydrate; MES monohydrate,≥99%(T); 2-(N MORPHOLINO) ETHANESULFONIC ACID, MONOHYDRATE

What is MES MONOHYDRATE with CAS 145224-94-8?

MES monohydrate is a kind of White crystalline powder. It is a biological buffer. MES Monohydrate, also known as 2-(4-Morpholinyl)ethanesulfonic acid hydrate, is a zwitterionic buffering agent widely used in biochemical and biological research. It belongs to the “Good’s buffers” family, known for providing stable pH control in the physiological range without interfering with most biochemical reactions.

MES Monohydrate Buffer Salt is especially valued for its minimal interference with metal ions, enzyme reactions, and UV absorbance, making it ideal for sensitive biological and biochemical systems.

Specification

ITEM	STANDARD	RESULT
Appearance	White crystalline Powder	Conform
Solubility (10% soln in H2O)	Clear	Conform
Color of a 1M Alkaline solution	Clear/colorless	Conform
Loss on drying	7-10%	8.95%
PH(1% soln in H2O, 25℃)	2.5-4.0	3.68
Pka(20℃)	6.02-6.25	6.14
Heavy metals(as Pb)	≤0.001%	<0.001%
Fe	≤0.001%	<0.001%
Chloride	≤0.01%	0.002%
Sulfate	≤0.01%	0.005%
Absorbance (250nm,10%)	≤0.05	0.016
Assay (by titn, dried basis)	≥99.0%	99.41%

Applications of MES Monohydrate (CAS 145224-94-8)

1. Biological and Biochemical Research

MES monohydrate is commonly used as a biological buffer in cell culture, protein purification, and enzyme assays. It helps maintain a stable pH in the physiological range (pH 5.5–6.7), ensuring optimal conditions for biological reactions.

2. Electrophoresis and Protein Studies

In electrophoresis systems, 2-(4-Morpholinyl)ethanesulfonic acid hydrate is used to prepare running and sample buffers that maintain consistent pH and ionic strength during protein separation and analysis.

3. Cell Culture Media and Diagnostic Reagents

MES monohydrate buffer salt is used to adjust and stabilize the pH in culture media and diagnostic formulations. Its low reactivity with metal ions and biomolecules ensures the integrity of test results.

4. Pharmaceutical and Biotechnological Applications

MES Monohydrate serves as a key buffering component in pharmaceutical formulations, bioprocessing, and analytical chemistry, providing reproducible conditions in manufacturing and testing processes.