HEPES CAS 7365-45-9

CAS: 7365-45-9
Purity: 99.5%MIN.
Molecular Formula: C8H18N2O4S
Molecular Weight: 238.3
EINECS: 230-907-9

Synonyms: HEPES2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONICACIDMOLECULARBIOLOGYGRADE; HEPES2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONICACID99+%; N-(2-HYDROXYETHYL)-PIPERAZINE-N’-2-ETHANESULFONICACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESUChemicalbookLFONICACID(HEPES-ACID)); Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz; 1-HeptaneSulfonicAcidSod.SaltGr; N-(2-Hydroxyethyl)Piperazine-N’-(3-EthanesulfonicAcid); N-(2-Hydroxyethyl)Piperazine-N’-(2-EthanesulfonicAcid)(Hepes); Hepes,LowSodium,FreeAcid

What is HEPES CAS 7365-45-9? HEPES CAS 7365-45-9 has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Description

ITEM	STANDARD
Appearance	white crystalline powder
Application	PH manager
PH value	5.0-6.5 (1Maqueous solution)
Fusing point	234-238 °C
Boiling point	408℃[at 101 325 Pa]
Packing density	560kg/m3
Density	1.07 g/mL at 20 °C
Storage condition	2-8°C

Application

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.